Sal

joined 3 years ago
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[–] Sal@mander.xyz 2 points 2 months ago (2 children)

I am using the integrated LED. Sometimes I use a UV flashlight to see fluorescence, and I would like to play with others. I work with spectroscopy and I have the intention of adding a bit of spectroscopy to the microscope, but I am still not there yet.

The camera adapter that I am using does contain some optics inside and has 2X magnification. It is one like this one: https://nl.aliexpress.com/item/32943613016.html

Microbehunter has a review on these: https://www.youtube.com/watch?v=Ju8rgeJr3bI

Roughly, if you use a 40x objective, the 2x adapter, and the camera is a DSLR with an APS-C sensor like mine (dimensions 23.6 mm x 15.7 mm), then the image that you capture with the camera corresponds to approximately the sensor dimensions / (objective multiplier x adapter multiplier) = 295 micron x 196 micron in this case. In practice it is good to use a microscope ruler for accurate dimensions,

There are camera adapter designs with no optics that you can 3D print, but I have not tested those. What I have tested are tubes that you can 3D print that connect the camera to an objective directly without the need for a microscope. This is can be useful for macro photography but the microscope is a lot more comfortable to use for general microscopy. This is what I mean: https://www.youtube.com/watch?v=nuZ_JptlqYE

[–] Sal@mander.xyz 34 points 2 months ago (1 children)

I think that for mid-levels of fame you can find a mixture of musical skill and self-marketing/entrepreneurship.

But as you go up the ladder of fame you get to the rungs where money is used to pay for an artist's exposure. The artist becomes an investor's asset and the "skill" of building fame arguably belongs to the investors / management team. And it is not so much skill as much as it is the power of capital to purchase attention.

[–] Sal@mander.xyz 2 points 2 months ago (4 children)

I just noticed that the one I have is 380T, not 350T! The 380T is slightly more expensive but allegedly has a bit better of a view on the edges. Here is a review from microbe hunter: https://www.microbehunter.com/swift-sw380t/

It comes with a dust cover, so no worries ;)

I did let mine sit for a while at first. There is a learning curve to getting things on the plane and focused with good lighting, and the first several times using the microscope was a whole activity in itself, so I would only use for a special occasion when I wanted to spend time learning. Over time it becomes easier to prepare samples, keep the optics clean, and to know what you are looking at. The microscope becomes a useful tool rather than an activity, and then it is less likely to collect dust because the opportunities to use it become plenty.

Not trying to bias you though, take your time! Haha

[–] Sal@mander.xyz 3 points 2 months ago

Next time you will be better prepared. This opportunity seems to happen every 2,000 years or so, don't worry, just a bit of patience and keep stone tablets on hand.

[–] Sal@mander.xyz 3 points 2 months ago (1 children)

7?! Was it a single day hike? They are beautiful, great shots!

[–] Sal@mander.xyz 2 points 2 months ago* (last edited 2 months ago) (6 children)

EDIT: I just checked and I actually have the SW380T, not 350T. It is similar but slightly better from what I can find: https://www.microbehunter.com/swift-sw380t/

Interesting! I ran a quick search to look into what you might see. I found a paper titled "ESTABLISHING NORMAL FECAL FLORA IN WILD AUSTRALIAN PASSERINE BIRDS BY USE OF THE FECAL GRAM STAIN, and in that paper they find no parasites in the feces sampled and mostly gram-positive bacterial with cocci shape. Yeasts were found some rarely. To actually identify the bacteria they use DNA sequencing. Other than the bacteria, if the sample is fresh, you might be able to find crystals (like uric acid), and epithelial cells... If the sample is a bit older you would also be able to identify microorganisms that feed on it.

The version I have at home is a SWIFT SW350T. It costs ~360 € in the EU. It has the eye pieces and third a tube for mounting a camera. For mounting the camera I purchased an adapter separately for my Nikon camera (it mounts to the DSLR camera as a regular lens and then the tube is inserted through the top port of the microscope). The SW350 (without 'T') is slightly cheaper at $300 and it missing the top port, but it is still possible to mount a camera through an eye piece. Having the third one is just a bit more convenient as you can look through the microscope while the camera is mounted.

I use it as a hobby. I have worked with a few fancy microscope types in a research setting, but then the kinds of samples I have looked at have been very restricted.

[–] Sal@mander.xyz 1 points 2 months ago

Thanks for the details!

Biofilm is exactly where I tend to find them Yeah, I got that from the video description :D

This video doesn’t have stentors, but it is of my thickest biofilm, a lot of stentors were found in the same sample: https://youtu.be/T3Bbg-ObTok

That video looks really nice! At first I thought it might be phase contrast, but I see from the description that you got creative. Nice job

[–] Sal@mander.xyz 2 points 2 months ago (2 children)

Ooh, interesting to learn! I think it is because I only collected a few samples from the wild when I first got my microscope and still had no idea of how prepare the samples or what I was looking it, so if there was a stentor in there I probably did not notice...

I have practiced since then with fresh samples of things that I find around my apartment and things that I culture - and the occasional lichen. Tomorrow I will go find some samples from outside - I will get some biofilm from a pond too and look for stentor.

[–] Sal@mander.xyz 2 points 2 months ago (1 children)

Very nice photos!

[–] Sal@mander.xyz 2 points 2 months ago (1 children)

At first I thought I was looking at some kind of radiator and that a very tiny pigeon was standing on the valve.

[–] Sal@mander.xyz 3 points 2 months ago (2 children)

Did you hand it over two stone tablets?

[–] Sal@mander.xyz 5 points 2 months ago (1 children)

Yes, it is possible!

The objective I used to take the photo is the '40x' objective. If looking through a 10x eyepiece (which is common) the total magnification when looking through the eyepiece is 400x.

If you look through a 4x objective and a 10x eyepiece then the magnification is 40x. The blood cells are visible at this magnification but they look quite small. I am specifying in case the '40x' was understood as 40x total magnification.

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submitted 9 months ago* (last edited 9 months ago) by Sal@mander.xyz to c/herpetology@mander.xyz
 

One of my favorite frogs! Spent some time specifically looking for it. Managed to find two near Cobá, in Quintana Roo (in the Yucatán peninsula). This frog hides in holes in lime stone and tree trunks, and makes use of its flat head to block the entrance. This type of defense is known as phragmosis.

Some other shots:

 

Found this anole in Yucatan. I am not sure of its exact species - probably an Anolis sp..

Anoles often sleep at the tips of leaves and twigs. My guess is that this is a strategy to prevent nocturnal predators sneaking up on them through the branches without making its bed vibrate. When woken up they can quickly drop down.

 

These are quite common in Yucatan, Mexico.

The leatherleaf slugs belong to the family Veronicellidae. This particular one could be Sarasinula plebeia, but it is not so easy to definitively ID these.

I originally identified this species a few years ago from the description on this website, but since then they have added an update stating that my original source is also unsure on this one.

UPDATE: It seems that IDing certain slugs by pictures isn’t a good idea. In 2024 when pictures on this page were uploaded to iNaturalist, another user suggested a different species in the genus Leidyula, and then user “deneb16,” a mollusk specialist at UNAM, Mexico’s main university, added the comment that all Mexican species of the family this slug belongs to can’t be identified without dissecting their sexual organs. The family, she agrees, is the Leatherleaf Slug Family, the Veroncellidae.

So, I am not 100% of the species, but it is a leatherleaf slug.

 

This was near the entrance to a mangrove boat tour. Tourists and locals feed the raccoons so they are very used to humans and will even follow them to try to get food.

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submitted 9 months ago* (last edited 9 months ago) by Sal@mander.xyz to c/entomology@mander.xyz
 

This bird poop caught my attention because it looked a bit too perfectly shaped, and when I looked at it closer its orange osmeteria came out.

Searching online, I suspect that this might be Papilio cresphontes. However, I see a lot of variation in the photos of Papilio cresphontes and so I am not sure if this the correct ID.

Found in Yucatan, Mexico.

 

adult greenhouse frog

baby greenhouse frog

Found many of these frogs under a wet trash bag in a back yard in Mérida, Yucatán. I made use of a handheld flash with a remote trigger for lighting, and a Sigma 105 mm as my macro lens.

These frogs do not seem to match with any of the local frogs reported in Julian C. Lee's field guide to the amphibians and reptiles of the maya world.

From a reverse image search, I mostly found images of the green house frog Eleutherodactylus planirostris. Eleutherodactylus planirostris is native to Cuba and the Bahamas, and is often introduced with plants that come from green houses in those areas. These frogs go from tadpole to frog while still inside of the egg, which explains why the baby frogs are so small.

The visual aspect, the incredibly small baby frogs, and the fact that they were found in a back yard in the city with greenhouse plants, all lead me to conclude that it is likely Eleutherodactylus planirostris.

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